An ARIMNet project
WP coordinator: INRA-LEG
Other partners involved: CSIC, IAV, INRAT, CRRGC, ARC, ITQB
Objectives:
- Development of reliable screening methods for resistance to drought and salt stresses in legumes
- Validation of drought and salt tolerance in present chickpea, faba bean, lentil and pea material
- Development of genotypes with improved drought tolerance
Methodology and study materials
T3.1: Experimental conditions for investigating pea response to drought and salinity will be established (INRA-LEG), in collaboration with INRAT (year 1). The High Troughput Plant Phenotyping Platform (PPHD) located in Dijon (France), allows the detailed analysis of up to 2000 plants grown under various climatic conditions, through dynamically, but non-destructively characterization by image analysis. RGB visible imaging methodologies will allow gathering details on the architecture of the shoot and root system, while Near Infra Red imaging will allow the measure of soil and plant water status.
T3.2: Screening methods for abiotic stress tolerance will be chosen (year 1). To this purpose, indirect methods (non-destructive and low-cost) such as gas-exchange measurements, chlorophyll fluorescence and canopy temperature depression (CTD) will be tested as indirect, rapid screening methods. They will be confronted to fine phenotyping methods obtained on the PPHD. Indeed, detailed information on plant water status in reaction to limited water availability or in reaction to salinity will be aquired automatically on the platform. In parallel, non-invasive methods will complete the phenotyping such as measurements of the photosynthetic activity, or N2 fixation, as well as destructive methods including membrane permeability and carbon isotope discrimination (Delta C13). The indirect method the most representative of the fine phenotyping method will be chosen for the next tasks.
T3.3: Experimental conditions, and the indirect method for screening for drought and salt tolerance will be adapted from T3.1 and T3.2, respectively, to screen chickpea (CRRGC, INRAT), common bean (ITQB), faba bean (CRRGC, IAV and INRAT), lentil (INRAT), pea (CSIC, CRRGC and INRA-LEG,) genotypes for tolerance to drought and salt stresses. Field trials will comprise one site across years 2 and 3, with two treatments, irrigated vs. drought, both under near field (rain-out shelter) and field condition (natural drought). In addition, plants in controlled-environment chambers will be subjected to either non-stress or drought / salt-stress conditions.
T3.4: Mechanisms underlying pea resistance to drought will be identified (years 2 and 3) using an ecophysiological framework which includes water-, nitrogen- and carbon- fluxes analyses within the plant, on several contrasted genotypes inoculated with Rhizobium strains shown to be drought-tolerant (INRA-LEG and INRAT). As a complementary approach, the understanding of physiological and molecular basis underlying salinity tolerance will also be studied, with a particular focus on ROS production, and oxidative damage (ARC).
Deliverables:
D3.1: Standardized experimental conditions for drought and salt tolerance
D3.2: Rapid methods for screening for drought and salinity tolerance
D3.3: Identification of sources of resistance to drought and salinity (on various species, and in combination with particular Rhizobium strains)
Other partners involved: CSIC, IAV, INRAT, CRRGC, ARC, ITQB
Objectives:
- Development of reliable screening methods for resistance to drought and salt stresses in legumes
- Validation of drought and salt tolerance in present chickpea, faba bean, lentil and pea material
- Development of genotypes with improved drought tolerance
Methodology and study materials
T3.1: Experimental conditions for investigating pea response to drought and salinity will be established (INRA-LEG), in collaboration with INRAT (year 1). The High Troughput Plant Phenotyping Platform (PPHD) located in Dijon (France), allows the detailed analysis of up to 2000 plants grown under various climatic conditions, through dynamically, but non-destructively characterization by image analysis. RGB visible imaging methodologies will allow gathering details on the architecture of the shoot and root system, while Near Infra Red imaging will allow the measure of soil and plant water status.
T3.2: Screening methods for abiotic stress tolerance will be chosen (year 1). To this purpose, indirect methods (non-destructive and low-cost) such as gas-exchange measurements, chlorophyll fluorescence and canopy temperature depression (CTD) will be tested as indirect, rapid screening methods. They will be confronted to fine phenotyping methods obtained on the PPHD. Indeed, detailed information on plant water status in reaction to limited water availability or in reaction to salinity will be aquired automatically on the platform. In parallel, non-invasive methods will complete the phenotyping such as measurements of the photosynthetic activity, or N2 fixation, as well as destructive methods including membrane permeability and carbon isotope discrimination (Delta C13). The indirect method the most representative of the fine phenotyping method will be chosen for the next tasks.
T3.3: Experimental conditions, and the indirect method for screening for drought and salt tolerance will be adapted from T3.1 and T3.2, respectively, to screen chickpea (CRRGC, INRAT), common bean (ITQB), faba bean (CRRGC, IAV and INRAT), lentil (INRAT), pea (CSIC, CRRGC and INRA-LEG,) genotypes for tolerance to drought and salt stresses. Field trials will comprise one site across years 2 and 3, with two treatments, irrigated vs. drought, both under near field (rain-out shelter) and field condition (natural drought). In addition, plants in controlled-environment chambers will be subjected to either non-stress or drought / salt-stress conditions.
T3.4: Mechanisms underlying pea resistance to drought will be identified (years 2 and 3) using an ecophysiological framework which includes water-, nitrogen- and carbon- fluxes analyses within the plant, on several contrasted genotypes inoculated with Rhizobium strains shown to be drought-tolerant (INRA-LEG and INRAT). As a complementary approach, the understanding of physiological and molecular basis underlying salinity tolerance will also be studied, with a particular focus on ROS production, and oxidative damage (ARC).
Deliverables:
D3.1: Standardized experimental conditions for drought and salt tolerance
D3.2: Rapid methods for screening for drought and salinity tolerance
D3.3: Identification of sources of resistance to drought and salinity (on various species, and in combination with particular Rhizobium strains)
WP 3 - Studies relevant for breeding for major abiotic constraints