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An ARIMNet project
WP coordinator: CSIC

Other partners involved: INRA, INRAT, CRRGC, INRAM, ARC, ITQB

Objectives
- Development of reliable screening methods for the most relevant biotic stresses: Ascochyta blights, broomrapes, rusts, fusarium wilts and chocolate spot both under field and growth chamber conditions.
- Identification of sources of resistance
- Characterisation of the resistance mechanisms at the cellular, physiological and molecular level
- Multilocation studies on stability of resistance

Methodology and study materials
T2.1: During year 1 different screening methods will be validated to establish a standard method that is quick, convenient and reliable for breeding.
T2.2: Adapted varieties as well as germplasm collections from each country will be tested, under both field and growth chamber conditions, to identify sources of resistance to major diseases (ascochyta blights, rusts, broomrapes, fusarium wilts and chocolate spot).
T2.3: Stability of the resistance (about 40 entries per crop) will be studied in multi-environment experiments, and in growth chamber against a range of isolates of contrasting virulence during years 2 and 3. Partner 1 (CSIC) will be responsible to coordinate the ring tests. Participating partners will be CSIC, CRRGC, INRAM, INRAT and ARC for chocolate spot (B. fabae); CSIC, CRRGC, INRA-IGEPP, INRAM and INRAT for ascochyta blights (Ascochyta rabiei, A. fabae, A. lentis and M. pinodes); CSIC, CRRGC, INRAM, INRAT and ARC for broomrapes (Orobanche crenata and O. foetida); CSIC, CRRGC, INRAM, INRAT and UKAFR for rusts (Uromyces appendiculatus, U. ciceris-arietini, U. viciae-fabae and U. pisi); and CSIC, CRRGC, INRA-IGEPP, INRAM, INRAT and ARC for fusariums (F. oxysporum ff.ssp.).
T2.4: Interesting accessions will be crossed with elite cultivars varieties by each partner to produce pre-breeding material that will be submitted to national breeding programmes that should be completed outside this project.

Deliverables
D2.1: Rapid and reliable screening methods suitable for selection and breeding
D2.2: Development of breeding material with improved resistance tested at a wide range of environments and pathogen populations
D2.3: Catalogue of sources of resistance

WP 2 - Studies relevant for breeding for resistance to major biotic constraints